Document Type
Poster
Session Format
Graduate Research Poster (no oral presentation)
Location
Magnolia Ballroom
Publication Date
Spring 3-27-2024
Faculty Advisor
Dave Bachoon
Start Date
27-3-2024 10:00 AM
End Date
27-3-2024 10:50 AM
Abstract
Pensacola, Florida (USA), has a thriving coastal community that depends on its coastal areas for recreation, shellfish harvesting, and fisheries. However, increasing levels of fecal pollution pose a potential threat to the water quality of these coastal systems. Identifying the source and abundance of contaminants in these environments is critical to controlling fecal pollution in these areas. This study aimed to use Microbial Source Tracking techniques to determine the source of pollution in five sites (Bayou Texar Lower, Willard Norris Road Creek, Clear Creek Boat Ramp @ 87, Quinette River Boat Ramp and Pensacola Bay Bridge) in the Pensacola area. DNA was extracted from water samples and then analyzed using PCR-based molecular Microbial Source Tracking techniques to determine the presence of human (HF-183-1), dog (BacCan), and avian (CP1) fecal pollution in these sites. Human-specific fecal bacteria were detected in Bayou Texar Lower, Willard Norris Road Creek, and Pensacola Bay Bridge sites. Dog-specific fecal bacteria were detected in Willard Norris Road Creek, Clear Creek Boat Ramp, and Pensacola Bay Bridge. There was no detection of avian-specific fecal bacteria in any of the sites. Human and dog fecal pollution detection in these coastal areas was attributed to urban developmental activities.
Recommended Citation
McCullers, Logan; Nicholas, Jodel; and Bachoon, Dave, "Molecular Detection of Human and Dog Fecal Pollution in Pensacola, Florida" (2024). Graduate Research Showcase. 139.
https://kb.gcsu.edu/grposters/139