Event Title

Evaluation of culture conditions and enumeration methods of enteric bacteria isolated from ground beef

Major

Biology

Faculty Mentor

Indiren Pillay

Abstract

An established protocol for the enrichment of Escherichia coli using E.coli Media with Novobiocin (ECM) was compared to a more convenient universal enrichment media, Universal Pre-enrichment Broth (UPB). Additionally, microbial growth after various incubation times of both media at 24, 36 and 48 hours was compared. There is no significant difference between ECM and UPB for the enrichment of E. coli isolated from raw meat sources. However, ECM appears to be better at enriching and isolating presumptive pathogens, such as E.coli O157:H7. Furthermore, we compared a commercially available Colilert-18 enumeration system to a novel, ready to use, Compact Dryª system. Because Colilert-18 is routinely used for water analysis and Compact Dryª is designed for the enumeration of coliforms from raw materials and environmental surfaces, we were therefore interested to see which of these systems would be most effective using samples collected from raw meat sources. Preliminary results indicate that for highly concentrated meat samples, Compact Dryª media may be more efficient at obtaining coliform and E.coli counts.

Session Name:

Poster Presentation Session #2 - Poster #60

Start Date

10-4-2015 12:15 PM

End Date

10-4-2015 1:00 PM

Location

HSB 3rd Floor Student Commons

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Apr 10th, 12:15 PM Apr 10th, 1:00 PM

Evaluation of culture conditions and enumeration methods of enteric bacteria isolated from ground beef

HSB 3rd Floor Student Commons

An established protocol for the enrichment of Escherichia coli using E.coli Media with Novobiocin (ECM) was compared to a more convenient universal enrichment media, Universal Pre-enrichment Broth (UPB). Additionally, microbial growth after various incubation times of both media at 24, 36 and 48 hours was compared. There is no significant difference between ECM and UPB for the enrichment of E. coli isolated from raw meat sources. However, ECM appears to be better at enriching and isolating presumptive pathogens, such as E.coli O157:H7. Furthermore, we compared a commercially available Colilert-18 enumeration system to a novel, ready to use, Compact Dryª system. Because Colilert-18 is routinely used for water analysis and Compact Dryª is designed for the enumeration of coliforms from raw materials and environmental surfaces, we were therefore interested to see which of these systems would be most effective using samples collected from raw meat sources. Preliminary results indicate that for highly concentrated meat samples, Compact Dryª media may be more efficient at obtaining coliform and E.coli counts.