Research Publication Title

The Formation of Robust Biofilms and Kaolin’s Sorption of Them

Major

Biology

Faculty Mentor(s)

Dr. Andrei L. Barkovskii

Keywords

Kaolin, Biofilms, Sorption, Serratia, Pseudomonas aeruginosa, Pseudomonas chlororaphis, Brevundimonas

Abstract

Biofilms create problems for medical, food, and water treatment industries. Kaolin products have a potential to be used against biofilms. Biofilms of Serratia nematodophila, Serratia marcescens, Pseudomonas aeruginosa, Pseudomonas chlororaphis, and Brevundimonas sp. have been created (37˚C, 24 hours, 96-well microplates) and evaluated using a modified micro-photometer approach. The three bacteria which produced the greatest amount of biofilm, Brevundimonas sp, Pseudomonas aeruginosa, and Pseudomonas chlororaphis, were for the trials. All trials have been performed in triplicates. Various substrates, including metal screws, plastic piping, and rocks, were incubated in a broth containing either a single bacteria, or a combination of three. The bacteria and biofilms were incubated for one month, after which the biofilms’ robustness and the ability of the kaolin to remove them was tested. The results of this and other trials suggested that P. aeruginosa can be used for testing the efficacy of kaolin products against biofilms. In addition, the efficacy of the above products was tested against naturally formed biofilms of, at this time, unknown composition. Obtained results suggest that selected kaolin products can become effective tools against biofilms.

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The Formation of Robust Biofilms and Kaolin’s Sorption of Them

Biofilms create problems for medical, food, and water treatment industries. Kaolin products have a potential to be used against biofilms. Biofilms of Serratia nematodophila, Serratia marcescens, Pseudomonas aeruginosa, Pseudomonas chlororaphis, and Brevundimonas sp. have been created (37˚C, 24 hours, 96-well microplates) and evaluated using a modified micro-photometer approach. The three bacteria which produced the greatest amount of biofilm, Brevundimonas sp, Pseudomonas aeruginosa, and Pseudomonas chlororaphis, were for the trials. All trials have been performed in triplicates. Various substrates, including metal screws, plastic piping, and rocks, were incubated in a broth containing either a single bacteria, or a combination of three. The bacteria and biofilms were incubated for one month, after which the biofilms’ robustness and the ability of the kaolin to remove them was tested. The results of this and other trials suggested that P. aeruginosa can be used for testing the efficacy of kaolin products against biofilms. In addition, the efficacy of the above products was tested against naturally formed biofilms of, at this time, unknown composition. Obtained results suggest that selected kaolin products can become effective tools against biofilms.