Document Type


Session Format

Graduate Research Poster (no oral presentation)


Magnolia Ballroom

Publication Date


Faculty Advisor

Andrei Barkovskii

Start Date

27-3-2024 10:00 AM

End Date

27-3-2024 10:50 AM


In our recent study, a wide array of virulence genes carried by Vibrio Harveyi clade pathogens has been detected in Georgia aquaculture waters and sediments. Identification of their carriers was attempted with qPCR and a set of previously published species-specific primers.

Reported as discriminating between species of Harveyi clade, the Vp-toxR primer (V. parahaemolyticus) cross-reacted with V. alginolyticus and V. campbellii. The Va-gyrB primer (Vibrio alginolyticus) was species-specific but revealed a very low sensitivity and cannot be used for detecting this pathogen at environmentally relevant concentrations. The rpoA primer (V. harveyi and V. campbellii) exhibited high selectivity for the above species and a lack of cross-reactions to other species of this clade.

A set of new primers has been obtained, and the VP 1155272 primer (V. alginolyticus) revealed a high species-specificity with a satisfactory detection limit. Testing and verifying the primers for other Harveyi clade species from the above set are in progress.

Our results call for more careful testing of published species-specific primers and qPCR parameters; and possibly for re-evaluation of published data obtained with the above primers. This ongoing study has highlighted the importance of evaluating published primers for their specificity and detection limits even if they have been previously reported as efficient primers for the detection and quantitation of Vibrio Harveyi clade species.



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