Research Publication Title

Combating Bacterial Keratitis with Common Disinfectants and Antibiotic Treatments

Presenter Information

Ariana BachoonFollow

Major

Biology

Faculty Mentor

Samuel Mutiti and Dave Bachoon

Keywords

Bacteria, eye, Keratitis, infections, disinfectants

Abstract

Bacterial Keratitis is usually caused by Pseudomonas aeruginosa,Streptococcus pneumoniaeand Staphylococcus aureus. Propper care, storage and cleaning of contact lenses is very important in preventing Bacterial Keratitis. The aim of this project was to evaluate the effectiveness of several popular contact lens cleaning and storage solutions at disinfecting bacteria on contact lens and determining the most appropriate antibiotic to use for treating eye infections by P. aeruginosa,S. pneumoniaeand S. aureus. Soft contact lenses were contaminated with Pseudomonas aeruginosaand Staphylococcus aureusfor 48 hrs. The lens was treated with saline (control), Equate, Bio true, Opti free and Blink for 24 hrs. ATP determination using Hygienic ATP test swabs indicated that Equate and Bio true were the most effective at reducing or inhibiting bacterial growth. Kirby Bower test indicated that Tetracycline was the most effective against P. aeruginosaand streptomycin, tetracycline and erythromycin were most effective against S. aureus. S. pneumoniaewas inhibited by all the antibiotics tested

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Combating Bacterial Keratitis with Common Disinfectants and Antibiotic Treatments

Bacterial Keratitis is usually caused by Pseudomonas aeruginosa,Streptococcus pneumoniaeand Staphylococcus aureus. Propper care, storage and cleaning of contact lenses is very important in preventing Bacterial Keratitis. The aim of this project was to evaluate the effectiveness of several popular contact lens cleaning and storage solutions at disinfecting bacteria on contact lens and determining the most appropriate antibiotic to use for treating eye infections by P. aeruginosa,S. pneumoniaeand S. aureus. Soft contact lenses were contaminated with Pseudomonas aeruginosaand Staphylococcus aureusfor 48 hrs. The lens was treated with saline (control), Equate, Bio true, Opti free and Blink for 24 hrs. ATP determination using Hygienic ATP test swabs indicated that Equate and Bio true were the most effective at reducing or inhibiting bacterial growth. Kirby Bower test indicated that Tetracycline was the most effective against P. aeruginosaand streptomycin, tetracycline and erythromycin were most effective against S. aureus. S. pneumoniaewas inhibited by all the antibiotics tested

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