SDS PAGE technique plays an essential role in the study of protein. However, this analytical method occasionally suffers from limitations such as poor separation, signal reduction, or total absence of target bands. This research aims to develop a protocol to improve the consistency of the technique while quantifying 10-12kDa parvalbumin proteins. It is a major fish allergen associated with neurodegenerative diseases, yet, the amount of parvalbumin in different kinds of fish has not been published. In this research, proteins in flounder, white, catfish and tilapia were extracted with PBS and quantified with Bradford assay and SDS PAGE. The results supported that the 0.5g fish muscle/ 1750uL PBS extraction was the most optimized and that white fish had the highest amount of proteins, followed by tilapia, flounder, and catfish.
"Optimization of SDS PAGE Analysis of Parvalbumin Protein in Fish,"
Undergraduate Research: Vol. 1
, Article 4.
Available at: https://kb.gcsu.edu/undergraduateresearch/vol1/iss2/4