Event Title
Purification of Sec15p using Kluyveromyces lactis
Faculty Mentor
Yen Kang France
Keywords
Yen Kang France
Abstract
The goal of our project is to identify novel interactors of a protein Sec15p, a member of the Exocyst complex that mediates post-Golgi secretory vesicle targeting in budding yeast. Due to low abundance of endogenous Sec15p for biochemical experiments, attempts were made to overexpress Sec15p within S. cerevisiae cells, but the overexpression resulted in cellular toxicity. We have been attempting to circumvent the challenge by expressing Sec15p in a different yeast strain, Kluyveromyces lactis where the proprietary vector allowed the secretion of Sec15p out into the media instead of accumulating intracellularly. Full length and truncated versions of SEC15 gene have been cloned for the experiments, and currently, we are working to optimize the expression and purification of Sec15p for the affinity chromatography.
Session Name:
Poster Presentation Session #2 - Poster #14
Start Date
4-4-2014 12:15 PM
End Date
4-4-2014 1:00 PM
Location
HSB 3rd Floor Student Commons
Purification of Sec15p using Kluyveromyces lactis
HSB 3rd Floor Student Commons
The goal of our project is to identify novel interactors of a protein Sec15p, a member of the Exocyst complex that mediates post-Golgi secretory vesicle targeting in budding yeast. Due to low abundance of endogenous Sec15p for biochemical experiments, attempts were made to overexpress Sec15p within S. cerevisiae cells, but the overexpression resulted in cellular toxicity. We have been attempting to circumvent the challenge by expressing Sec15p in a different yeast strain, Kluyveromyces lactis where the proprietary vector allowed the secretion of Sec15p out into the media instead of accumulating intracellularly. Full length and truncated versions of SEC15 gene have been cloned for the experiments, and currently, we are working to optimize the expression and purification of Sec15p for the affinity chromatography.