Event Title
Characterization and Genomic Analysis of a Subcluster K2 Mycobacteriophage
Faculty Mentor
Indiren Pillay
Keywords
Indiren Pillay
Abstract
A novel mycobacteriophage was isolated from environmental samples collected in Baldwin County, Georgia. Purified phage lysates were imaged by Transmission Electron Microscopy. The isometric capsids of approximately 60 nm in diameter, and non-contractile tails of approximately 190 nm in length, indicate Siphoviridae morphology. The phage DNA was isolated, purified and characterized by restriction endonuclease digestion. The complete phage genome was then sequenced via ion torrent sequencing at the Pittsburgh Bacteriophage Institute. Preliminary analysis and comparison with existing phage data in the PhagesDB.org mycobacteriophage database indicate it is a K2 Subcluster phage. Using DNA analysis software, genomic annotation of ‘Milly’ to identify genes and their function was undertaken. The genome is 58,211 base pairs long with 68.3% GC content. Preliminary annotations reveal approximately 97 genes in this genome. This genetic analysis of ‘Milly’ is confirmed as a K2 phage and contributes more information to our understanding of the Cluster K phages.
Session Name:
Poster Presentation Session #2 - Poster #22
Start Date
4-4-2014 12:15 PM
End Date
4-4-2014 1:00 PM
Location
HSB 3rd Floor Student Commons
Characterization and Genomic Analysis of a Subcluster K2 Mycobacteriophage
HSB 3rd Floor Student Commons
A novel mycobacteriophage was isolated from environmental samples collected in Baldwin County, Georgia. Purified phage lysates were imaged by Transmission Electron Microscopy. The isometric capsids of approximately 60 nm in diameter, and non-contractile tails of approximately 190 nm in length, indicate Siphoviridae morphology. The phage DNA was isolated, purified and characterized by restriction endonuclease digestion. The complete phage genome was then sequenced via ion torrent sequencing at the Pittsburgh Bacteriophage Institute. Preliminary analysis and comparison with existing phage data in the PhagesDB.org mycobacteriophage database indicate it is a K2 Subcluster phage. Using DNA analysis software, genomic annotation of ‘Milly’ to identify genes and their function was undertaken. The genome is 58,211 base pairs long with 68.3% GC content. Preliminary annotations reveal approximately 97 genes in this genome. This genetic analysis of ‘Milly’ is confirmed as a K2 phage and contributes more information to our understanding of the Cluster K phages.