Event Title
Genetic Assessment of Cambarus truncatus (Oconee Burrowing Crayfish) Populations
Faculty Mentor
Yen Kang France
Keywords
Yen Kang France
Abstract
Cambarus truncatus Hobbs is a freshwater crayfish species known from only 15 localities in the Oconee River system of Georgia. Despite the fact that the various populations of C. truncatus have become increasingly isolated due to anthropogenic forces and are considered by many authorities to be threatened, almost nothing is known with regards to its life history or genetic diversity. The goal of this project is to assess the genetic diversity of C. truncatus among different populations using molecular phylogenetic analysis and to gain insight into its dispersal capability. The gene regions selected for analysis are the mitochondrial genes, 16s and Cytochrome Oxidase I (COI). Genomic PCR was performed using total genomic DNA extracted from acquired specimens, and all gene makers were sequenced analysis. Bayesian and Likelihood analysis indicates that there is low genetic diversity between the sampled populations, and that gene flow is limited.
Session Name:
Biological and Environmental Sciences IV
Start Date
4-4-2014 1:15 PM
End Date
4-4-2014 2:15 PM
Location
HSB 207
Genetic Assessment of Cambarus truncatus (Oconee Burrowing Crayfish) Populations
HSB 207
Cambarus truncatus Hobbs is a freshwater crayfish species known from only 15 localities in the Oconee River system of Georgia. Despite the fact that the various populations of C. truncatus have become increasingly isolated due to anthropogenic forces and are considered by many authorities to be threatened, almost nothing is known with regards to its life history or genetic diversity. The goal of this project is to assess the genetic diversity of C. truncatus among different populations using molecular phylogenetic analysis and to gain insight into its dispersal capability. The gene regions selected for analysis are the mitochondrial genes, 16s and Cytochrome Oxidase I (COI). Genomic PCR was performed using total genomic DNA extracted from acquired specimens, and all gene makers were sequenced analysis. Bayesian and Likelihood analysis indicates that there is low genetic diversity between the sampled populations, and that gene flow is limited.