Date of Award
Summer 7-27-2023
Document Type
Thesis
Degree Name
Master of Biological Science (MBioSci)
Department
Biological Science
First Advisor
Dr. Ellen France
Second Advisor
Dr. Ashok Hegde
Third Advisor
Dr. Richard Adams
Abstract
Twenty-seven years ago, Saccharomyces cerevisiae became the first eukaryote to have its full genome sequenced, thus leading to the discovery that 30% of genes related to human diseases are orthologous to those in S. cerevisiae. Since then, 75% of the proteome has had its localization classified, and we sought to fill the remaining gaps of knowledge by identifying the localization of three proteins: Fsh3, Gid10, and Ade13, which function as a serine hydrolase, ubiquitin ligase, and adenylosuccinate lyase, respectively. To visualize cellular localization, we used a C-terminal GFP tagging strategy and subsequent fluorescence microscopy. Through colocalization analyses, we identified the cellular localization of Ade13 to be mitochondrial while Fsh3 and Gid10 localize to distinct puncta which are predicted to be peroxisomal. Interestingly, we observed that the localization of Gid10 changes depending on the glucose availability in the media. As each investigated yeast protein has human orthologues whose malfunctions lead to diseases in humans, our findings lay important basic foundations for future studies comparing S. cerevisiae and human proteins in conserved processes.
Recommended Citation
Barr, Lewis; Adams, Richard H.; and France, Ellen PhD, "Examining Protein Localization via Fluorescence Microscopy in Saccharomyces cerevisiae" (2023). Biology Theses. 31.
https://kb.gcsu.edu/biology/31