Degradation of Estrogen: An NMR Study
Abstract
Can1H-NMR spectroscopy be used to watch estrogen degrade in real time? In this work,two forms of estrogen, 17_-estradiol(E2)and_-ethinyl estradiol (EE2), are being investigated usingNMR. The overall goal wasto determine if NMR can be used to monitor the degradation of estrogens intoother products. It is known that EE2 makes its way into natural water sources, and this is problematic dueto the feminizingeffectsthatestrogen compounds can haveon fish and other aquatic life.Literature has shown that E2 and EE2 can be degraded in water using both biodegradation and photodegradation to form less harmful products.In research doneby ourcollaborators(S. Mutiti, et al.) inenvironmental sciences, the samples of estrogen have been purposelydegraded in an effort to determine the products formed. However, analysis of the degradation products has proven challenging. Hence NMR was chosen as a potential method to monitor the degradation.After determining the optimumNMR solvent fordistinguishingEE2 and E2, 1H-NMR spectroscopy was used to monitor the degradation of an EE2 sample over a given time.Results will be reported in this work.
Session Name:
Poster Presentation Session #1 - Poster #63
Start Date
10-4-2015 11:30 AM
End Date
10-4-2015 12:15 PM
Location
HSB 3rd Floor Student Commons
Degradation of Estrogen: An NMR Study
HSB 3rd Floor Student Commons
Can1H-NMR spectroscopy be used to watch estrogen degrade in real time? In this work,two forms of estrogen, 17_-estradiol(E2)and_-ethinyl estradiol (EE2), are being investigated usingNMR. The overall goal wasto determine if NMR can be used to monitor the degradation of estrogens intoother products. It is known that EE2 makes its way into natural water sources, and this is problematic dueto the feminizingeffectsthatestrogen compounds can haveon fish and other aquatic life.Literature has shown that E2 and EE2 can be degraded in water using both biodegradation and photodegradation to form less harmful products.In research doneby ourcollaborators(S. Mutiti, et al.) inenvironmental sciences, the samples of estrogen have been purposelydegraded in an effort to determine the products formed. However, analysis of the degradation products has proven challenging. Hence NMR was chosen as a potential method to monitor the degradation.After determining the optimumNMR solvent fordistinguishingEE2 and E2, 1H-NMR spectroscopy was used to monitor the degradation of an EE2 sample over a given time.Results will be reported in this work.