Localization of Pat1b After Adenovirus Infection
Abstract
Adenovirus is the largest non-enveloped double stranded virus that causes a range of diseases from the common cold to more fatal conditions in the immunocompromised. During the infectious cycle, Adenovirus can shut off host cell protein synthesis and use it for viral replication. There are 57 human serotypes of the adenovirus and many animal serotypes. Serotype 5 (Ad 5) in particular has been shown to disrupt processing bodies, also known as p-bodies, during the viral life cycle. A few of the p-bodies known to be disrupted are Ddx6, Lsm1, and Ge-1. A fourth p-body protein is Pat1b, which is as a scaffolding protein involved in p-body formation and also acts as a translational regulator. Pat1b has not been observed during an infection of Adenovirus, consequently we decided to visualize the p-body along-side other cellular proteins using immunofluorescence microscopy to identify its location and pervasiveness. Pat1b was observed to co-localize with Ddx6 in aggresomes, and that the number of Pat1b foci during and Ad5 infection, when compared to an uninfected cell, increase. Otherwise, the number of Ddx6 foci during an Ad5 infection decrease. Currently we are testing whether the increase in Pat1b foci is due to overexpression or merely redistribution of Pat1b.
Localization of Pat1b After Adenovirus Infection
Adenovirus is the largest non-enveloped double stranded virus that causes a range of diseases from the common cold to more fatal conditions in the immunocompromised. During the infectious cycle, Adenovirus can shut off host cell protein synthesis and use it for viral replication. There are 57 human serotypes of the adenovirus and many animal serotypes. Serotype 5 (Ad 5) in particular has been shown to disrupt processing bodies, also known as p-bodies, during the viral life cycle. A few of the p-bodies known to be disrupted are Ddx6, Lsm1, and Ge-1. A fourth p-body protein is Pat1b, which is as a scaffolding protein involved in p-body formation and also acts as a translational regulator. Pat1b has not been observed during an infection of Adenovirus, consequently we decided to visualize the p-body along-side other cellular proteins using immunofluorescence microscopy to identify its location and pervasiveness. Pat1b was observed to co-localize with Ddx6 in aggresomes, and that the number of Pat1b foci during and Ad5 infection, when compared to an uninfected cell, increase. Otherwise, the number of Ddx6 foci during an Ad5 infection decrease. Currently we are testing whether the increase in Pat1b foci is due to overexpression or merely redistribution of Pat1b.