Event Title

Sol-Gel Encapsulation of Horseradishperoxidase

Presenter Information

Benjamin Ottoson

Faculty Mentor

Catrena Lisse

Keywords

Catrena Lisse

Abstract

Sol-gels are porous and optically transparent glass monoliths that have various functions. Their applications and development in biomedicine have shown promise for new biosensors and artificial organs. Sol-gels can be synthesized from various starting materials including tetramethoxysilane (TMOS) and tetraethoxysilane (TEOS). These various starting materials create different pore sizes that will allow a comparison of which process is a more suitable environment for enzyme encapsulation. Several types of sol-gels doped with Horseradish Peroxidase (HRP) are synthesized and investigated. Effectiveness is gauged via UV-Vis spectroscopy and analysis of the affinity of HRP for hydrogen peroxide is measured using concentration values obtained from the Beer-Lambert law. The syntheses and characterization of these materials are highlighted in this presentation.

Session Name:

Chemistry I

Start Date

4-4-2014 10:15 AM

End Date

4-4-2014 11:15 AM

Location

HSB 144

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Apr 4th, 10:15 AM Apr 4th, 11:15 AM

Sol-Gel Encapsulation of Horseradishperoxidase

HSB 144

Sol-gels are porous and optically transparent glass monoliths that have various functions. Their applications and development in biomedicine have shown promise for new biosensors and artificial organs. Sol-gels can be synthesized from various starting materials including tetramethoxysilane (TMOS) and tetraethoxysilane (TEOS). These various starting materials create different pore sizes that will allow a comparison of which process is a more suitable environment for enzyme encapsulation. Several types of sol-gels doped with Horseradish Peroxidase (HRP) are synthesized and investigated. Effectiveness is gauged via UV-Vis spectroscopy and analysis of the affinity of HRP for hydrogen peroxide is measured using concentration values obtained from the Beer-Lambert law. The syntheses and characterization of these materials are highlighted in this presentation.