Event Title
Sol-Gel Encapsulation of Horseradishperoxidase
Faculty Mentor
Catrena Lisse
Keywords
Catrena Lisse
Abstract
Sol-gels are porous and optically transparent glass monoliths that have various functions. Their applications and development in biomedicine have shown promise for new biosensors and artificial organs. Sol-gels can be synthesized from various starting materials including tetramethoxysilane (TMOS) and tetraethoxysilane (TEOS). These various starting materials create different pore sizes that will allow a comparison of which process is a more suitable environment for enzyme encapsulation. Several types of sol-gels doped with Horseradish Peroxidase (HRP) are synthesized and investigated. Effectiveness is gauged via UV-Vis spectroscopy and analysis of the affinity of HRP for hydrogen peroxide is measured using concentration values obtained from the Beer-Lambert law. The syntheses and characterization of these materials are highlighted in this presentation.
Session Name:
Chemistry I
Start Date
4-4-2014 10:15 AM
End Date
4-4-2014 11:15 AM
Location
HSB 144
Sol-Gel Encapsulation of Horseradishperoxidase
HSB 144
Sol-gels are porous and optically transparent glass monoliths that have various functions. Their applications and development in biomedicine have shown promise for new biosensors and artificial organs. Sol-gels can be synthesized from various starting materials including tetramethoxysilane (TMOS) and tetraethoxysilane (TEOS). These various starting materials create different pore sizes that will allow a comparison of which process is a more suitable environment for enzyme encapsulation. Several types of sol-gels doped with Horseradish Peroxidase (HRP) are synthesized and investigated. Effectiveness is gauged via UV-Vis spectroscopy and analysis of the affinity of HRP for hydrogen peroxide is measured using concentration values obtained from the Beer-Lambert law. The syntheses and characterization of these materials are highlighted in this presentation.