Event Title

Distribution of Escherichia coli Phylotypes from Herbivores in Kenya, Africa using Multiplex Polymerase Chain Reaction

Presenter Information

Brian Mears

Faculty Mentor

Dave Bachoon

Keywords

Dave Bachoon

Abstract

The purpose of this study was to investigate the diversity of E. coli strains carried by herbivores in Kenya and identify animals that act as a reservoir for pathogenic E. coli strains. E. coli can be grouped into one of four phylogenetic groups (A. B1, B2, D) based upon certain gene combinations. E. coli was isolated from fecal samples of 12 species of animals in Africa. PCR was used to quickly group E. coli strains on the presence of two genes (chuA and yjaA) and an anonymous DNA fragment (TSPE4.C2). The study included: gazelle, oryx, sheep, impala, water buck, cattle, dik-dik, eland, buffalo, giraffe, Thomson’s gazelle, and hartebeest. The results indicated that dik-dik, hartebeest, buffalo, Thomson’s gazelle, and gazelle carried mainly non-pathogenic strains of E. coli. The other animals carried both pathogenic and non-pathogenic strains.

Session Name:

Biological and Environmental Sciences III

Start Date

4-4-2014 10:15 AM

End Date

4-4-2014 11:15 AM

Location

HSB 207

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Apr 4th, 10:15 AM Apr 4th, 11:15 AM

Distribution of Escherichia coli Phylotypes from Herbivores in Kenya, Africa using Multiplex Polymerase Chain Reaction

HSB 207

The purpose of this study was to investigate the diversity of E. coli strains carried by herbivores in Kenya and identify animals that act as a reservoir for pathogenic E. coli strains. E. coli can be grouped into one of four phylogenetic groups (A. B1, B2, D) based upon certain gene combinations. E. coli was isolated from fecal samples of 12 species of animals in Africa. PCR was used to quickly group E. coli strains on the presence of two genes (chuA and yjaA) and an anonymous DNA fragment (TSPE4.C2). The study included: gazelle, oryx, sheep, impala, water buck, cattle, dik-dik, eland, buffalo, giraffe, Thomson’s gazelle, and hartebeest. The results indicated that dik-dik, hartebeest, buffalo, Thomson’s gazelle, and gazelle carried mainly non-pathogenic strains of E. coli. The other animals carried both pathogenic and non-pathogenic strains.