Event Title

Rapid Detection of Helicobacter pylori and Escherichia coli O157:H7 in the Tidally Influenced Point Judith Pond of Rhode Island.

Presenter Information

Ralph Rozier

Faculty Mentor

Dave Bachoon

Keywords

Dave Bachoon

Abstract

The water quality at 41 sites from Point Judith Pound was assessed for fecal pollution and the presence of human pathogenic bacteria. The water samples from 30 of the 41 sites exceeded USEPA regulatory limit for safe recreational and shellfish harvesting use. Quantitative polymerase chain reaction (qPCR) was used to determine the presence of Escherichia coli O157:H7 and Helicobacter pylori at the sites. The pathogenic bacteria H. pylori were detected in eight sites but E. coli O157:H7 was undetected at any of our sampling sites. Overall, higher levels of H. pylori were detected in the eelgrass (50%) compared to the water column and sediment samples. Microbial Source Tracking using Bifidobacterium adolescentis, and the Bacteroides marker HF183 detected the presence of human fecal at seven locations on the pond. Enterococci was not always a good indicator for the presence of pathogenic bacteria such as H. pylori.

Session Name:

Biological and Environmental Sciences III

Start Date

4-4-2014 10:15 AM

End Date

4-4-2014 11:15 AM

Location

HSB 207

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Apr 4th, 10:15 AM Apr 4th, 11:15 AM

Rapid Detection of Helicobacter pylori and Escherichia coli O157:H7 in the Tidally Influenced Point Judith Pond of Rhode Island.

HSB 207

The water quality at 41 sites from Point Judith Pound was assessed for fecal pollution and the presence of human pathogenic bacteria. The water samples from 30 of the 41 sites exceeded USEPA regulatory limit for safe recreational and shellfish harvesting use. Quantitative polymerase chain reaction (qPCR) was used to determine the presence of Escherichia coli O157:H7 and Helicobacter pylori at the sites. The pathogenic bacteria H. pylori were detected in eight sites but E. coli O157:H7 was undetected at any of our sampling sites. Overall, higher levels of H. pylori were detected in the eelgrass (50%) compared to the water column and sediment samples. Microbial Source Tracking using Bifidobacterium adolescentis, and the Bacteroides marker HF183 detected the presence of human fecal at seven locations on the pond. Enterococci was not always a good indicator for the presence of pathogenic bacteria such as H. pylori.